› 5ukw

X-ray diffraction
2.65Å resolution

Crystal structure of human Glucose 6-phosphate Dehydrogenase mutant (A277C) complexed with G6P

Released:
DOI: 10.2210/pdb5ukw/pdb
PDB entry 5ukw coloured by chain, front view.
PDB entry 5ukw coloured by chain, side view.
PDB entry 5ukw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mutations in the tetramer interface of human glucose-6-phosphate dehydrogenase reveals kinetic differences between oligomeric states.
Ranzani AT, Cordeiro AT
FEBS Lett 591 1278-1284 (2017)
PMID: 28370139

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 56.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11413 (Residues: 29-511; Coverage: 94%)
Gene name: G6PD
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BG6 1 x BG6
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: F222
Unit cell:
a: 59.099Å b: 177.817Å c: 216.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Treatment strategies for glucose-6-phosphate dehydrogenase deficiency: past and future perspectives.
Garcia et al. (2021)
1 more

1 mention without citation

Crystal structure of Leishmania donovani glucose 6-phosphate dehydrogenase reveals a unique N-terminal domain.
Berneburg et al. (2022)