› 5uld

X-ray diffraction
2.78Å resolution

Structure and function of the divalent anion/Na+ symporter from Vibrio cholerae and a humanized variant

Released:
DOI: 10.2210/pdb5uld/pdb
PDB entry 5uld coloured by chain, front view.
PDB entry 5uld coloured by chain, side view.
PDB entry 5uld coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Primary publication:
Structure and function of the divalent anion/Na+ symporter from Vibrio cholerae and a humanized variant.
Nie R, Stark S, Symersky J, Kaplan RS, Lu M
Nat Commun 8 15009 (2017)
PMID: 28436435

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate transporter-like domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 445 amino acids
Theoretical weight: 47.63 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KNE0 (Residues: 18-462; Coverage: 96%)
Gene name: VC_A0025
Sequence domains: Citrate transporter

Ligands and Environments

2 bound ligands:
Ligand NA 8 x NA
Ligand CIT 4 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 106.087Å b: 101.535Å c: 168.882Å
α: 90° β: 99.73° γ: 90°
R-values:
R R work R free
0.253 0.252 0.268
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Consequences of NaCT/SLC13A5/mINDY deficiency: good versus evil, separated only by the blood-brain barrier.
Kopel et al. (2021)
3 more

9 mentions without citation

Drosophila INDY and Mammalian INDY: Major Differences in Transport Mechanism and Structural Features despite Mostly Similar Biological Functions.
Jaramillo-Martinez et al. (2021)
8 more