› 5ulg

X-ray diffraction
2.1Å resolution

Crystal structure of RPE65 in complex with MB-008 and palmitate

Released:
DOI: 10.2210/pdb5ulg/pdb
PDB entry 5ulg coloured by chain, front view.
PDB entry 5ulg coloured by chain, side view.
PDB entry 5ulg coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Rational Tuning of Visual Cycle Modulator Pharmacodynamics.
Kiser PD, Zhang J, Badiee M, Kinoshita J, Peachey NS, Tochtrop GP, Palczewski K
J Pharmacol Exp Ther 362 131-145 (2017)
PMID: 28476927

Function and Biology Details

Reactions catalysed: 
An all-trans-retinyl ester + H(2)O = 11-cis-retinol + a fatty acid
Lutein = meso-zeaxanthin
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173309 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinoid isomerohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 61.04 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q28175 (Residues: 1-533; Coverage: 100%)
Gene name: RPE65
Sequence domains: Retinal pigment epithelial membrane protein

Ligands and Environments

5 bound ligands:
Ligand FE2 2 x FE2
Ligand PLM 2 x PLM
Ligand 8E4 2 x 8E4
Ligand PG4 2 x PG4
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P65
Unit cell:
a: 175.319Å b: 175.319Å c: 86.653Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.176 0.207

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Citations

4 review citations

Ten Years of Knowledge of Nano-Carrier Based Drug Delivery Systems in Ophthalmology: Current Evidence, Challenges, and Future Prospective.
Zhang et al. (2021)
3 more

1 mention without citation

Rational Tuning of Visual Cycle Modulator Pharmacodynamics.
Kiser et al. (2017)