› 5umx

X-ray diffraction
1.59Å resolution

Crystal structure of TnmS3 in complex with riboflavin

Released:
DOI: 10.2210/pdb5umx/pdb
PDB entry 5umx coloured by chain, front view.
PDB entry 5umx coloured by chain, side view.
PDB entry 5umx coloured by chain, top view.
Source organism: Streptomyces sp. CB03234
Primary publication:
Resistance to Enediyne Antitumor Antibiotics by Sequestration.
Chang CY, Yan X, Crnovcic I, Annaval T, Chang C, Nocek B, Rudolf JD, Yang D, Babnigg G, Joachimiak A, Phillips GN, Shen B
Cell Chem Biol 25 1075-1085.e4 (2018)
PMID: 29937405

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102867 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.15 KDa
Source organism: Streptomyces sp. CB03234
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A125SA29 (Residues: 1-124; Coverage: 100%)
Gene names: AMK26_32110, tnmS3
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

1 bound ligand:
Ligand RBF 2 x RBF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 45.059Å b: 60.915Å c: 85.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.193
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Crossroads of Antibiotic Resistance and Biosynthesis.
Wencewicz TA. (2019)
2 more

1 mention without citation

Resistance to Enediyne Antitumor Antibiotics by Sequestration.
Chang et al. (2018)