› 5upu

X-ray diffraction
2.9Å resolution

Crystal Structure of the Catalytic Domain of the Inosine Monophosphate Dehydrogenase from Mycobacterium tuberculosis in the presence of TBK6

Released:
DOI: 10.2210/pdb5upu/pdb
PDB entry 5upu coloured by chain, front view.
PDB entry 5upu coloured by chain, side view.
PDB entry 5upu coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Kim Y, Makowska-Grzyska M, Maltseva N, Mulligan R, Gu M, Sacchettini J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 407 amino acids
Theoretical weight: 41.64 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WKI7 (Residues: 1-125, 256-529; Coverage: 75%)
Gene names: MTCY78.17, Rv3411c, guaB, guaB2
Sequence domains: IMP dehydrogenase / GMP reductase domain
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand IMP 1 x IMP
Ligand 6Q9 1 x 6Q9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4
Unit cell:
a: 88.614Å b: 88.614Å c: 85.056Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.174 0.235
Expression system: Escherichia coli BL21(DE3)

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