› 5uq4

X-ray diffraction
2.2Å resolution

Crystal structure of Heme-Degrading Protein Rv3592 from Mycobacterium tuberculosis - heme free with cleaved protein

Released:
DOI: 10.2210/pdb5uq4/pdb
Model geometry
Fit model/data
PDB entry 5uq4 coloured by chain, front view.
PDB entry 5uq4 coloured by chain, side view.
PDB entry 5uq4 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Chang C, Chhor G, Jedrzejczak R, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Protoheme + 3 reduced acceptor + 3 O(2) = mycobilin a + Fe(2+) + 3 acceptor + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161743 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme oxygenase (mycobilin-producing) Chains: A, B
Molecule details ›
Chains: A, B
Length: 108 amino acids
Theoretical weight: 11.47 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P9WKH3 (Residues: 1-105; Coverage: 100%)
Gene names: Rv3592, mhuD
Sequence domains: Antibiotic biosynthesis monooxygenase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P61
Unit cell:
a: 38.134Å b: 38.134Å c: 238.638Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.172 0.223
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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