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› 5uqk

X-ray diffraction
1.85Å resolution

Clostridium difficile toxin A (TcdA) glucosyltransferase domain in complex with U2F

Released:
DOI: 10.2210/pdb5uqk/pdb
Model geometry
Fit model/data
PDB entry 5uqk coloured by chain, front view.
PDB entry 5uqk coloured by chain, side view.
PDB entry 5uqk coloured by chain, top view.
Source organism: Clostridioides difficile
Primary publication:
Clostridium difficile toxin glucosyltransferase domains in complex with a non-hydrolyzable UDP-glucose analogue.
Alvin JW, Lacy DB
J Struct Biol 198 203-209 (2017)
PMID: 28433497

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucosyltransferase TcdA Chain: A
Molecule details ›
Chain: A
Length: 558 amino acids
Theoretical weight: 64.71 KDa
Source organism: Clostridioides difficile
Expression system: Priestia megaterium
UniProt:
  • Canonical: P16154 (Residues: 1-544; Coverage: 20%)
Gene names: tcdA, toxA
Sequence domains:
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

2 bound ligands:
Ligand U2F 1 x U2F
Ligand MN 1 x MN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P65
Unit cell:
a: 142.81Å b: 142.81Å c: 66.074Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.207
Expression system: Priestia megaterium

Quick links

Citations

1 review citation

The role of toxins in Clostridium difficile infection.
Chandrasekaran et al. (2017)
5 other articles

1 mention without citation

Catalytic DxD motif caged in Asx-turn and Met-aromatic interaction attenuates the pathogenic glycosylation of SseK2/NleB2 effectors.
Koh et al. (2022)