› 5ur7

X-ray diffraction
2Å resolution

Crystal structure of engineered CCL20 disulfide locked dimer

Released:
DOI: 10.2210/pdb5ur7/pdb
PDB entry 5ur7 coloured by chain, front view.
PDB entry 5ur7 coloured by chain, side view.
PDB entry 5ur7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Protein engineering of the chemokine CCL20 prevents psoriasiform dermatitis in an IL-23-dependent murine model.
Getschman AE, Imai Y, Larsen O, Peterson FC, Wu X, Rosenkilde MM, Hwang ST, Volkman BF
Proc Natl Acad Sci U S A 114 12460-12465 (2017)
PMID: 29109267

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160286 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-C motif chemokine 20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 70 amino acids
Theoretical weight: 8.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P78556 (Residues: 27-96; Coverage: 100%)
Gene names: CCL20, LARC, MIP3A, SCYA20
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
Ligand ACT 5 x ACT
Ligand IPA 1 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P61
Unit cell:
a: 71.679Å b: 71.679Å c: 71.561Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.16 0.197
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Modulation of the CCR6-CCL20 Axis: A Potential Therapeutic Target in Inflammation and Cancer.
Ranasinghe et al. (2018)
8 more

3 mentions without citation

The marriage of chemokines and galectins as functional heterodimers.
von Hundelshausen et al. (2021)
2 more