› 5uv1

X-ray diffraction
2.4Å resolution

Crystal Structure of (+)-Limonene Synthase Complexed with 2-Fluorogeranyl Diphosphate

Released:
DOI: 10.2210/pdb5uv1/pdb
PDB entry 5uv1 coloured by chain, front view.
PDB entry 5uv1 coloured by chain, side view.
PDB entry 5uv1 coloured by chain, top view.
Source organism: Citrus sinensis
Primary publication:
Structural Characterization of Early Michaelis Complexes in the Reaction Catalyzed by (+)-Limonene Synthase from Citrus sinensis Using Fluorinated Substrate Analogues.
Kumar RP, Morehouse BR, Matos JO, Malik K, Lin H, Krauss IJ, Oprian DD
Biochemistry 56 1716-1725 (2017)
PMID: 28272876

Function and Biology Details

Reaction catalysed: 
Geranyl diphosphate = (+)-(4R)-limonene + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103338 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(R)-limonene synthase 1, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 607 amino acids
Theoretical weight: 70.44 KDa
Source organism: Citrus sinensis
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1C9J6A7 (Residues: 1-607; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 3 x MN
Ligand 0FV 1 x 0FV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P41212
Unit cell:
a: 85.51Å b: 85.51Å c: 215.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.236
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural and Chemical Biology of Terpenoid Cyclases.
Christianson DW. (2017)
3 more

2 mentions without citation

Predictive Engineering of Class I Terpene Synthases Using Experimental and Computational Approaches.
Leferink et al. (2022)
1 more