› 5uwg

X-ray diffraction
2.56Å resolution

The crystal structure of Fz4-CRD in complex with palmitoleic acid

Released:
DOI: 10.2210/pdb5uwg/pdb
PDB entry 5uwg coloured by chain, front view.
PDB entry 5uwg coloured by chain, side view.
PDB entry 5uwg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Wnt5a promotes Frizzled-4 signalosome assembly by stabilizing cysteine-rich domain dimerization.
DeBruine ZJ, Ke J, Harikumar KG, Gu X, Borowsky P, Williams BO, Xu W, Miller LJ, Xu HE, Melcher K
Genes Dev 31 916-926 (2017)
PMID: 28546512

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Frizzled-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 14.15 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9ULV1 (Residues: 40-164; Coverage: 25%)
Gene name: FZD4
Sequence domains: Fz domain
Structure domains: Frizzled cysteine-rich domain

Ligands and Environments

2 bound ligands:
Ligand PAM 1 x PAM
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2221
Unit cell:
a: 103.452Å b: 109.373Å c: 76.789Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.267
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Targeting signalling pathways and the immune microenvironment of cancer stem cells - a clinical update.
Clara et al. (2020)
6 more

7 mentions without citation

Assembly and architecture of the Wnt/β-catenin signalosome at the membrane.
DeBruine et al. (2017)
6 more