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› 5uxa

X-ray diffraction
1.95Å resolution

Crystal structure of macrolide 2'-phosphotransferase MphB from Escherichia coli

Released:
DOI: 10.2210/pdb5uxa/pdb
Model geometry
Fit model/data
PDB entry 5uxa coloured by chain, front view.
PDB entry 5uxa coloured by chain, side view.
PDB entry 5uxa coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The evolution of substrate discrimination in macrolide antibiotic resistance enzymes.
Pawlowski AC, Stogios PJ, Koteva K, Skarina T, Evdokimova E, Savchenko A, Wright GD
OpenAccess logo Nat Commun 9 112 (2018)
PMID: 29317655

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-128457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32553 (Residues: 1-302; Coverage: 100%)
Gene names: EXX87_25395, HVY77_26855, mph(B), mphB, pO103_99
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

1 bound ligand:
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2221
Unit cell:
a: 67.276Å b: 116.684Å c: 92.574Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.151 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The Role of Antibiotic-Target-Modifying and Antibiotic-Modifying Enzymes in Mycobacterium abscessus Drug Resistance.
Luthra et al. (2018)
6 more

1 mention without citation

The evolution of substrate discrimination in macrolide antibiotic resistance enzymes.
Pawlowski et al. (2018)