› 5uxh

X-ray diffraction
2.41Å resolution

Structure of Human POFUT1 in complex with GDP-fucose

Released:
DOI: 10.2210/pdb5uxh/pdb
PDB entry 5uxh coloured by chain, front view.
PDB entry 5uxh coloured by chain, side view.
PDB entry 5uxh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human POFUT1, its requirement in ligand-independent oncogenic Notch signaling, and functional effects of Dowling-Degos mutations.
McMillan BJ, Zimmerman B, Egan ED, Lofgren M, Xu X, Hesser A, Blacklow SC
Glycobiology 27 777-786 (2017)
PMID: 28334865

Function and Biology Details

Reaction catalysed: 
GDP-beta-L-fucose + [protein]-(L-serine/L-threonine) = GDP + [protein]-3-O-(alpha-L-fucosyl)-(L-serine/L-threonine)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GDP-fucose protein O-fucosyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 41.87 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9H488 (Residues: 24-384; Coverage: 99%)
Gene names: FUT12, KIAA0180, POFUT1
Sequence domains: GDP-fucose protein O-fucosyltransferase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GFB 2 x GFB
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 50.23Å b: 133.92Å c: 70.01Å
α: 90° β: 104.13° γ: 90°
R-values:
R R work R free
0.243 0.24 0.292
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Protein O-fucosylation: structure and function.
Holdener et al. (2019)
7 more

5 mentions without citation

A perspective on structural and mechanistic aspects of protein O-fucosylation.
Lira-Navarrete et al. (2018)
4 more