› 5uz9

Electron Microscopy
3.4Å resolution

Cryo EM structure of anti-CRISPRs, AcrF1 and AcrF2, bound to type I-F crRNA-guided CRISPR surveillance complex

Released:
DOI: 10.2210/pdb5uz9/pdb
PDB entry 5uz9 coloured by chain, ensemble of 5 models, front view.
PDB entry 5uz9 coloured by chain, ensemble of 5 models, side view.
PDB entry 5uz9 coloured by chain, ensemble of 5 models, top view.
Source organisms:
Primary publication:
Structure Reveals Mechanisms of Viral Suppressors that Intercept a CRISPR RNA-Guided Surveillance Complex.
Chowdhury S, Carter J, Rollins MF, Golden SM, Jackson RN, Hoffmann C, Nosaka L, Bondy-Denomy J, Maxwell KL, Davidson AR, Fischer ER, Lander GC, Wiedenheft B
Cell 169 47-57.e11 (2017)
PMID: 28340349
Related structures: EMD-8624

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
PDBe Complex ID:
PDB-CPX-125823 (preferred)
Entry contents:
6 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (7 distinct):
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: C, D, E, F, G, H
Molecule details ›
Chains: C, D, E, F, G, H
Length: 341 amino acids
Theoretical weight: 37.45 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM1 (Residues: 2-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
Anti-CRISPR protein Acr30-35 Chains: I, J
Molecule details ›
Chains: I, J
Length: 77 amino acids
Theoretical weight: 8.69 KDa
Source organism: Pseudomonas phage JBD30
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: L7P7M1 (Residues: 2-78; Coverage: 99%)
Gene name: JBD30_035
Sequence domains: Anti-CRISPR protein Acr30-35/AcrF1
Structure domains: Viral Envelope Glycoprotein; domain 2
Anti-CRISPR protein 30 Chain: K
Molecule details ›
Chain: K
Length: 96 amino acids
Theoretical weight: 10.76 KDa
Source organism: Casadabanvirus D3112
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6TM72 (Residues: 2-90; Coverage: 99%)
Gene name: orf30
CRISPR-associated endonuclease Cas6/Csy4 Chain: L
Molecule details ›
Chain: L
Length: 189 amino acids
Theoretical weight: 21.69 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
Structure domains: CRISPR-associated endoribonuclease Cas6/Csy4
CRISPR RNA (60-MER) Chain: M
Molecule details ›
Chain: M
Length: 60 nucleotides
Theoretical weight: 19.25 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-8624
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

Anti-CRISPR: discovery, mechanism and function.
Pawluk et al. (2018)
29 more

16 mentions without citation

Structures and Strategies of Anti-CRISPR-Mediated Immune Suppression.
Wiegand et al. (2020)
15 more