› 5uzp

X-ray diffraction
2.29Å resolution

Crystal structure of citrate synthase mutant A348G from homo sapiens

Released:
DOI: 10.2210/pdb5uzp/pdb
PDB entry 5uzp coloured by chain, front view.
PDB entry 5uzp coloured by chain, side view.
PDB entry 5uzp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comparative studies of Aspergillus fumigatus 2-methylcitrate synthase and human citrate synthase.
Schlachter CR, Klapper V, Radford T, Chruszcz M
Biol Chem 400 1567-1581 (2019)
PMID: 31141475

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate synthase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 462 amino acids
Theoretical weight: 51.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75390 (Residues: 28-466; Coverage: 94%)
Gene name: CS
Sequence domains: Citrate synthase, C-terminal domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand OAA 2 x OAA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 58.245Å b: 111.804Å c: 74.162Å
α: 90° β: 99.31° γ: 90°
R-values:
R R work R free
0.158 0.156 0.205
Expression system: Escherichia coli

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Citations

3 review citations

Pathogenicity and virulence of Aspergillus fumigatus.
Earle et al. (2023)
2 more