› 5v1m

X-ray diffraction
1.47Å resolution

Structure of human Usb1 with uridine 5'-monophosphate

Released:
DOI: 10.2210/pdb5v1m/pdb
PDB entry 5v1m coloured by chain, front view.
PDB entry 5v1m coloured by chain, side view.
PDB entry 5v1m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities.
Didychuk AL, Montemayor EJ, Carrocci TJ, DeLaitsch AT, Lucarelli SE, Westler WM, Brow DA, Hoskins AA, Butcher SE
Nat Commun 8 497 (2017)
PMID: 28887445

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U6 snRNA phosphodiesterase 1 Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BQ65 (Residues: 79-265; Coverage: 71%)
Gene names: C16orf57, Mpn1, USB1
Sequence domains: Uncharacterised conserved protein
Structure domains: Cyclic phosphodiesterase

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand GOL 6 x GOL
Ligand U5P 1 x U5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 42.512Å b: 53.219Å c: 46.638Å
α: 90° β: 107.04° γ: 90°
R-values:
R R work R free
0.161 0.159 0.194
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Modifications in small nuclear RNAs and their roles in spliceosome assembly and function.
Bohnsack et al. (2018)
5 more

3 mentions without citation

Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities.
Didychuk et al. (2017)
2 more