› 5v48

X-ray diffraction
3Å resolution

Soluble rabbit neprilysin in complex with thiorphan

Released:
DOI: 10.2210/pdb5v48/pdb
PDB entry 5v48 coloured by chain, front view.
PDB entry 5v48 coloured by chain, side view.
PDB entry 5v48 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Structures of soluble rabbit neprilysin complexed with phosphoramidon or thiorphan.
Labiuk SL, Sygusch J, Grochulski P
Acta Crystallogr F Struct Biol Commun 75 405-411 (2019)
PMID: 31204686

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139900 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neprilysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 696 amino acids
Theoretical weight: 79.48 KDa
Source organism: Oryctolagus cuniculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P08049 (Residues: 55-750; Coverage: 93%)
Gene name: MME
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand ZN 2 x ZN
Ligand TIO 2 x TIO
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 68.994Å b: 108.446Å c: 211.887Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.195 0.245
Expression system: Komagataella pastoris

Quick links

Citations

1 citations in other articles

Structural analysis of Mycobacterium tuberculosis M13 metalloprotease Zmp1 open states.
Liang et al. (2021)

3 mentions without citation

An In Silico Target Fishing Approach to Identify Novel Ochratoxin A Hydrolyzing Enzyme.
Dellafiora et al. (2020)
2 more