› 5v7l

X-ray diffraction
3.2Å resolution

PCNA mutant R61A/D63A Protein Defective in Gene Silencing

Released:
DOI: 10.2210/pdb5v7l/pdb
PDB entry 5v7l coloured by chain, front view.
PDB entry 5v7l coloured by chain, side view.
PDB entry 5v7l coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structures of PCNA mutant proteins defective in gene silencing suggest a novel interaction site on the front face of the PCNA ring.
Kondratick CM, Litman JM, Shaffer KV, Washington MT, Dieckman LM
PLoS One 13 e0193333 (2018)
PMID: 29499038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proliferating cell nuclear antigen Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 29.77 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P15873 (Residues: 1-258; Coverage: 100%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P213
Unit cell:
a: 123.181Å b: 123.181Å c: 123.181Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.251
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The replisome guides nucleosome assembly during DNA replication.
Zhang et al. (2020)
2 more

1 mention without citation

Crystal structures of PCNA mutant proteins defective in gene silencing suggest a novel interaction site on the front face of the PCNA ring.
Kondratick et al. (2018)