› 5vbg

X-ray diffraction
2.8Å resolution

Crystal Structure of full-length LpoA, Monoclinic form 1, from Haemophilus influenzae

Released:
DOI: 10.2210/pdb5vbg/pdb
PDB entry 5vbg coloured by chain, front view.
PDB entry 5vbg coloured by chain, side view.
PDB entry 5vbg coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Primary publication:
Structural analyses of the Haemophilus influenzae peptidoglycan synthase activator LpoA suggest multiple conformations in solution.
Sathiyamoorthy K, Vijayalakshmi J, Tirupati B, Fan L, Saper MA
J Biol Chem 292 17626-17642 (2017)
PMID: 28887305

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein activator LpoA Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 60.53 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
UniProt:
  • Canonical: P45299 (Residues: 33-575; Coverage: 99%)
Gene names: HI_1655, lpoA
Sequence domains: LppC putative lipoprotein

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 67.458Å b: 69.344Å c: 73.577Å
α: 90° β: 110.18° γ: 90°
R-values:
R R work R free
0.207 0.205 0.246
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Normal Mode Analysis as a Routine Part of a Structural Investigation.
Bauer et al. (2019)
7 other articles

1 mention without citation

Structural analyses of the Haemophilus influenzae peptidoglycan synthase activator LpoA suggest multiple conformations in solution.
Sathiyamoorthy et al. (2017)