› 5ve6

X-ray diffraction
2.95Å resolution

Crystal structure of Sugen kinase 223

Released:
DOI: 10.2210/pdb5ve6/pdb
PDB entry 5ve6 coloured by chain, front view.
PDB entry 5ve6 coloured by chain, side view.
PDB entry 5ve6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of SgK223 pseudokinase reveals novel mechanisms of homotypic and heterotypic association.
Patel O, Griffin MDW, Panjikar S, Dai W, Ma X, Chan H, Zheng C, Kropp A, Murphy JM, Daly RJ, Lucet IS
Nat Commun 8 1157 (2017)
PMID: 29079850

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183279 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inactive tyrosine-protein kinase PRAG1 Chain: A
Molecule details ›
Chain: A
Length: 492 amino acids
Theoretical weight: 54.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86YV5 (Residues: 932-1406; Coverage: 34%)
Gene names: PRAG1, SGK223
Sequence domains: Protein tyrosine and serine/threonine kinase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P43212
Unit cell:
a: 144.683Å b: 144.683Å c: 47.445Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.218 0.262
Expression system: Escherichia coli

Quick links

Citations

2 review citations

PseudoGTPase domains in p190RhoGAP proteins: a mini-review.
Stiegler et al. (2018)
1 more

7 mentions without citation

Prospects for pharmacological targeting of pseudokinases.
Kung et al. (2019)
6 more