› 5vfx

X-ray diffraction
2.81Å resolution

Structure of an accessory protein of the pCW3 relaxosome in complex with the origin of transfer (oriT) DNA

Released:
DOI: 10.2210/pdb5vfx/pdb
PDB entry 5vfx coloured by chain, front view.
PDB entry 5vfx coloured by chain, side view.
PDB entry 5vfx coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3.
Traore DAK, Wisniewski JA, Flanigan SF, Conroy PJ, Panjikar S, Mok YF, Lao C, Griffin MDW, Adams V, Rood JI, Whisstock JC
Nat Commun 9 3732 (2018)
PMID: 30213934

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119277 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TcpK Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 107 amino acids
Theoretical weight: 13.01 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1PLI2 (Residues: 2-102; Coverage: 99%)
Gene names: I9080_003465, NCTC10719_03121, pBeta2_00022, pCW3_0028, pCpb2-CP1_20, pNetB-NE10_20, pNetB_00021, pTet_021
oriT Chains: I, K, N, O
Molecule details ›
Chains: I, K, N, O
Length: 23 nucleotides
Theoretical weight: 7.11 KDa
Source organism: Clostridium perfringens
Expression system: Not provided
oriT Chains: J, L, M, P
Molecule details ›
Chains: J, L, M, P
Length: 23 nucleotides
Theoretical weight: 7.01 KDa
Source organism: Clostridium perfringens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 69.503Å b: 109.894Å c: 122.78Å
α: 90° β: 93.02° γ: 90°
R-values:
R R work R free
0.2 0.198 0.233
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 citation in other articles

Pathogenicity and virulence of Clostridium perfringens.
Mehdizadeh Gohari et al. (2021)
8 more

2 mentions without citation

Regulation of Gram-Positive Conjugation.
Kohler et al. (2019)
1 more