› 5vhm

Electron Microscopy
8.3Å resolution

Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle

Released:
DOI: 10.2210/pdb5vhm/pdb
PDB entry 5vhm coloured by chain, front view.
PDB entry 5vhm coloured by chain, side view.
PDB entry 5vhm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.
Lu Y, Wu J, Dong Y, Chen S, Sun S, Ma YB, Ouyang Q, Finley D, Kirschner MW, Mao Y
Mol Cell 67 322-333.e6 (2017)
PMID: 28689658
Related structures: EMD-8678

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131140 (preferred)
Entry contents:
8 distinct polypeptide molecules
Macromolecules (8 distinct):
26S proteasome non-ATPase regulatory subunit 10 Chain: G
Molecule details ›
Chain: G
Length: 223 amino acids
Theoretical weight: 24.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O75832 (Residues: 4-226; Coverage: 99%)
Gene name: PSMD10
Sequence domains: Ankyrin repeats (3 copies)
26S proteasome regulatory subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 266 amino acids
Theoretical weight: 29.75 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P35998 (Residues: 159-424; Coverage: 61%)
Gene names: MSS1, PSMC2
Sequence domains:
26S proteasome regulatory subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 266 amino acids
Theoretical weight: 29.9 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P62191 (Residues: 167-432; Coverage: 61%)
Gene name: PSMC1
Sequence domains:
26S proteasome regulatory subunit 6B Chain: D
Molecule details ›
Chain: D
Length: 262 amino acids
Theoretical weight: 29.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P43686 (Residues: 145-406; Coverage: 63%)
Gene names: MIP224, PSMC4, TBP7
Sequence domains:
26S proteasome regulatory subunit 10B Chain: E
Molecule details ›
Chain: E
Length: 262 amino acids
Theoretical weight: 29.43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P62333 (Residues: 128-389; Coverage: 67%)
Gene names: PSMC6, SUG2
Sequence domains:
26S proteasome regulatory subunit 6A Chain: F
Molecule details ›
Chain: F
Length: 267 amino acids
Theoretical weight: 29.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P17980 (Residues: 166-432; Coverage: 61%)
Gene names: PSMC3, TBP1
Sequence domains:
26S proteasome regulatory subunit 8 Chain: C
Molecule details ›
Chain: C
Length: 266 amino acids
Theoretical weight: 29.5 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P62195 (Residues: 130-395; Coverage: 66%)
Gene names: PSMC5, SUG1
Sequence domains:
26S proteasome non-ATPase regulatory subunit 2 Chain: f
Molecule details ›
Chain: f
Length: 848 amino acids
Theoretical weight: 93.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q13200 (Residues: 6-853; Coverage: 93%)
Gene names: PSMD2, TRAP2
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 8.3Å
Relevant EMDB volumes: EMD-8678
Expression system: Homo sapiens

Quick links

Citations

7 review citations

Cryo-EM for Small Molecules Discovery, Design, Understanding, and Application.
Scapin et al. (2018)
6 more

1 mention without citation

Conformational Landscape of the p28-Bound Human Proteasome Regulatory Particle.
Lu et al. (2017)