› 5vir

X-ray diffraction
1.7Å resolution

Crystal structure of probable nicotinate-nucleotide adenylyltransferase from Mycobacterium abscessus in complex with NADP and compound FOL0091

Released:
DOI: 10.2210/pdb5vir/pdb
PDB entry 5vir coloured by chain, front view.
PDB entry 5vir coloured by chain, side view.
PDB entry 5vir coloured by chain, top view.
Source organism: Mycobacteroides abscessus ATCC 19977
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable nicotinate-nucleotide adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 25.5 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1MMZ4 (Residues: 2-211; Coverage: 100%)
Gene names: MAB_1621, nadD
Sequence domains: Cytidylyltransferase-like
Structure domains: HUPs

Ligands and Environments

2 bound ligands:
Ligand 9CG 2 x 9CG
Ligand NAP 2 x NAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 63.38Å b: 116.11Å c: 55.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.199
Expression system: Escherichia coli BL21(DE3)

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