› 5vke

X-ray diffraction
2.37Å resolution

Open conformation of KcsA deep-inactivated

Released:
DOI: 10.2210/pdb5vke/pdb
PDB entry 5vke coloured by chain, front view.
PDB entry 5vke coloured by chain, side view.
PDB entry 5vke coloured by chain, top view.
Source organisms:
Primary publication:
The gating cycle of a K+ channel at atomic resolution.
Cuello LG, Cortes DM, Perozo E
Elife 6 (2017)
PMID: 29165243

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209487 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody Light Chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 23.41 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Antibody Heavy Chain Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.44 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 10.07 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 26-121; Coverage: 60%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand F09 1 x F09
Ligand 1EM 1 x 1EM
Ligand K 3 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: I4
Unit cell:
a: 156.195Å b: 156.195Å c: 74.172Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.228
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
5 more

10 mentions without citation

Structural mechanisms of transient receptor potential ion channels.
Cao E. (2020)
9 more