› 5vp7

X-ray diffraction
1.7Å resolution

Crystal structure of human KRAS G12A mutant in complex with GDP

Released:
DOI: 10.2210/pdb5vp7/pdb
PDB entry 5vp7 coloured by chain, front view.
PDB entry 5vp7 coloured by chain, side view.
PDB entry 5vp7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into the rearrangement of the switch I region in GTP-bound G12A K-Ras.
Xu S, Long BN, Boris GH, Chen A, Ni S, Kennedy MA
Acta Crystallogr D Struct Biol 73 970-984 (2017)
PMID: 29199977

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase KRas, N-terminally processed Chains: A, F
Molecule details ›
Chains: A, F
Length: 170 amino acids
Theoretical weight: 19.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01116 (Residues: 1-169; Coverage: 89%)
Gene names: KRAS, KRAS2, RASK2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand GDP 2 x GDP
Ligand MG 2 x MG
Ligand PGE 1 x PGE
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: R3
Unit cell:
a: 93.773Å b: 93.773Å c: 119.918Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.171 0.207
Expression system: Escherichia coli

Quick links

Citations

6 review citations

KRAS as a druggable target in NSCLC: Rising like a phoenix after decades of development failures.
Friedlaender et al. (2020)
5 more

2 mentions without citation

RAS Nanoclusters: Dynamic Signaling Platforms Amenable to Therapeutic Intervention.
Van et al. (2021)
1 more