› 5vqi

X-ray diffraction
2.5Å resolution

Nuclear transport of the Neurospora crassa NIT2 transcription factor is mediated by Importin-alpha

Released:
DOI: 10.2210/pdb5vqi/pdb
Model geometry
Fit model/data
PDB entry 5vqi coloured by chain, front view.
PDB entry 5vqi coloured by chain, side view.
PDB entry 5vqi coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
Nuclear transport of the Neurospora crassa NIT-2 transcription factor is mediated by importin-α.
Bernardes NE, Takeda AAS, Dreyer TR, Cupertino FB, Virgilio S, Pante N, Bertolini MC, Fontes MRM
Biochem J 474 4091-4104 (2017)
PMID: 29054975

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148520 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 495 amino acids
Theoretical weight: 53.6 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9C2K9 (Residues: 75-548; Coverage: 87%)
Gene name: 3H10.030
Sequence domains:
Nitrogen catabolic enzyme regulatory protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 13 amino acids
Theoretical weight: 1.58 KDa
Source organism: Neurospora crassa
Expression system: Not provided
UniProt:
  • Canonical: P19212 (Residues: 915-927; Coverage: 1%)
Gene names: NCU09068, amr, nit-2, pink

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P212121
Unit cell:
a: 45.034Å b: 63.706Å c: 183.635Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Regulation of nutrient utilization in filamentous fungi.
Kerkaert et al. (2023)
2 other articles