› 5vsa

X-ray diffraction
2Å resolution

Crystal structure of SsoPox AsA1 mutant (C258L-I261F-W263A)

Released:
DOI: 10.2210/pdb5vsa/pdb
PDB entry 5vsa coloured by chain, front view.
PDB entry 5vsa coloured by chain, side view.
PDB entry 5vsa coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase.
Jacquet P, Hiblot J, Daudé D, Bergonzi C, Gotthard G, Armstrong N, Chabrière E, Elias M
Sci Rep 7 16745 (2017)
PMID: 29196634

Function and Biology Details

Reaction catalysed: 
An aryl dialkyl phosphate + H(2)O = dialkyl phosphate + an aryl alcohol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aryldialkylphosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 314 amino acids
Theoretical weight: 35.59 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97VT7 (Residues: 1-314; Coverage: 100%)
Gene names: SSO2522, php
Sequence domains: Phosphotriesterase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

3 bound ligands:
Ligand FE2 4 x FE2
Ligand CO 4 x CO
Ligand GOL 8 x GOL
1 modified residue:
Ligand KCX 4 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 86.6Å b: 103.1Å c: 151.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.204
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biotechnology of extremely thermophilic archaea.
Straub et al. (2018)
5 more

2 mentions without citation

Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase.
Jacquet et al. (2017)
1 more