› 5vu5

X-ray diffraction
2.8Å resolution

TNA polymerase, apo

Released:
DOI: 10.2210/pdb5vu5/pdb
PDB entry 5vu5 coloured by chain, front view.
PDB entry 5vu5 coloured by chain, side view.
PDB entry 5vu5 coloured by chain, top view.
Source organism: Thermococcus kodakarensis
Primary publication:
Structural basis for TNA synthesis by an engineered TNA polymerase.
Chim N, Shi C, Sau SP, Nikoomanzar A, Chaput JC
Nat Commun 8 1810 (2017)
PMID: 29180809

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 774 amino acids
Theoretical weight: 90.13 KDa
Source organism: Thermococcus kodakarensis
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWU9 (Residues: 1-774; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 72.819Å b: 110.62Å c: 111.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.268 0.317
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Modified nucleic acids: replication, evolution, and next-generation therapeutics.
Duffy et al. (2020)
8 more

1 mention without citation

Structural basis for TNA synthesis by an engineered TNA polymerase.
Chim et al. (2017)