› 5vwq

X-ray diffraction
1.8Å resolution

E.coli Aspartate aminotransferase-(1R,3S,4S)-3-amino-4-fluorocyclopentane-1-carboxylic acid (FCP)

Released:
DOI: 10.2210/pdb5vwq/pdb
PDB entry 5vwq coloured by chain, front view.
PDB entry 5vwq coloured by chain, side view.
PDB entry 5vwq coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Selective Targeting by a Mechanism-Based Inactivator against Pyridoxal 5'-Phosphate-Dependent Enzymes: Mechanisms of Inactivation and Alternative Turnover.
Mascarenhas R, Le HV, Clevenger KD, Lehrer HJ, Ringe D, Kelleher NL, Silverman RB, Liu D
Biochemistry 56 4951-4961 (2017)
PMID: 28816437

Function and Biology Details

Reaction catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 396 amino acids
Theoretical weight: 43.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00509 (Residues: 1-396; Coverage: 100%)
Gene names: JW0911, aspC, b0928
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 4 x PMP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 78.897Å b: 84.904Å c: 88.029Å
α: 118.83° β: 90.1° γ: 89.59°
R-values:
R R work R free
0.164 0.162 0.192
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Design and Mechanism of GABA Aminotransferase Inactivators. Treatments for Epilepsies and Addictions.
Silverman RB. (2018)
10 other articles

1 mention without citation

Selective Targeting by a Mechanism-Based Inactivator against Pyridoxal 5'-Phosphate-Dependent Enzymes: Mechanisms of Inactivation and Alternative Turnover.
Mascarenhas et al. (2017)