› 5vx4

X-ray diffraction
1.55Å resolution

VP8* of a G2P[4] Human Rotavirus

Released:
DOI: 10.2210/pdb5vx4/pdb
PDB entry 5vx4 coloured by chain, front view.
PDB entry 5vx4 coloured by chain, side view.
PDB entry 5vx4 coloured by chain, top view.
Source organism: Rotavirus A
Primary publication:
Glycan recognition in globally dominant human rotaviruses.
Hu L, Sankaran B, Laucirica DR, Patil K, Salmen W, Ferreon ACM, Tsoi PS, Lasanajak Y, Smith DF, Ramani S, Atmar RL, Estes MK, Ferreon JC, Prasad BVV
Nat Commun 9 2631 (2018)
PMID: 29980685

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer capsid protein VP4 Chain: A
Molecule details ›
Chain: A
Length: 162 amino acids
Theoretical weight: 18.5 KDa
Source organism: Rotavirus A
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2VE61 (Residues: 54-213; Coverage: 58%)
Gene name: VP4
Sequence domains: Outer Capsid protein VP4 (Hemagglutinin) Concanavalin-like domain
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 34.33Å b: 56.59Å c: 37.31Å
α: 90° β: 93.52° γ: 90°
R-values:
R R work R free
0.173 0.171 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The Impact of Human Genetic Polymorphisms on Rotavirus Susceptibility, Epidemiology, and Vaccine Take.
Sharma et al. (2020)
8 more

4 mentions without citation

Structural Basis of Glycan Recognition of Rotavirus.
Sun et al. (2021)
3 more