› 5vxn

X-ray diffraction
3.38Å resolution

Structure of two RcsB dimers bound to two parallel DNAs.

Released:
DOI: 10.2210/pdb5vxn/pdb
PDB entry 5vxn coloured by chain, front view.
PDB entry 5vxn coloured by chain, side view.
PDB entry 5vxn coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for DNA Recognition by the Two-Component Response Regulator RcsB.
Filippova EV, Zemaitaitis B, Aung T, Wolfe AJ, Anderson WF
mBio 9 (2018)
PMID: 29487239

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119304 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulatory protein RcsB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 216 amino acids
Theoretical weight: 23.7 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMC7 (Residues: 1-216; Coverage: 100%)
Gene names: JW2205, b2217, rcsB
Sequence domains:
DNA (5'-D(*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*A)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 18 nucleotides
Theoretical weight: 5.56 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*GP*AP*TP*TP*TP*AP*GP*GP*AP*AP*AP*AP*AP*TP*CP*TP*TP*AP*GP*AP*TP*A)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 18 nucleotides
Theoretical weight: 5.46 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 74.116Å b: 113.231Å c: 76.868Å
α: 90° β: 115.37° γ: 90°
R-values:
R R work R free
0.202 0.198 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Epigenetics in Breast Cancer Therapy-New Strategies and Future Nanomedicine Perspectives.
Buocikova et al. (2020)
1 more

1 mention without citation

Structural Basis for DNA Recognition by the Two-Component Response Regulator RcsB.
Filippova et al. (2018)