› 5vyf

X-ray diffraction
2.9Å resolution

Structure of single chain Fel d 1 bound to a neutralizing antibody

Released:
DOI: 10.2210/pdb5vyf/pdb
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PDB entry 5vyf coloured by chain, front view.
PDB entry 5vyf coloured by chain, side view.
PDB entry 5vyf coloured by chain, top view.
Source organisms:
Primary publication:
Treating cat allergy with monoclonal IgG antibodies that bind allergen and prevent IgE engagement.
Orengo JM, Radin AR, Kamat V, Badithe A, Ben LH, Bennett BL, Zhong S, Birchard D, Limnander A, Rafique A, Bautista J, Kostic A, Newell D, Duan X, Franklin MC, Olson W, Huang T, Gandhi NA, Lipsich L, Stahl N, Papadopoulos NJ, Murphy AJ, Yancopoulos GD
OpenAccess logo Nat Commun 9 1421 (2018)
PMID: 29650949

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-286010 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.4 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 220 amino acids
Theoretical weight: 23.47 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Major allergen I polypeptide chain 1; Major allergen I polypeptide chain 2 Chains: C, F

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 74.985Å b: 131.48Å c: 148.357Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.259
Expression system: Homo sapiens

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Citations

49 review citations

IgE and IgG Antibodies as Regulators of Mast Cell and Basophil Functions in Food Allergy.
Kanagaratham et al. (2020)
48 more

4 mentions without citation

Structural Aspects of the Allergen-Antibody Interaction.
Pomés et al. (2020)
3 more

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