› 5vyj

X-ray diffraction
3.3Å resolution

Crystal structure of the photosynthetic phosphoenolpyruvate carboxylase isoenzyme from maize in complex with Gly

Released:
DOI: 10.2210/pdb5vyj/pdb
PDB entry 5vyj coloured by chain, front view.
PDB entry 5vyj coloured by chain, side view.
PDB entry 5vyj coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Identification of the allosteric site for neutral amino acids in the maize C4 isozyme of phosphoenolpyruvate carboxylase: The critical role of Ser-100.
González-Segura L, Mújica-Jiménez C, Juárez-Díaz JA, Güémez-Toro R, Martinez-Castilla LP, Muñoz-Clares RA
J Biol Chem 293 9945-9957 (2018)
PMID: 29743237

Function and Biology Details

Reaction catalysed: 
Phosphate + oxaloacetate = H(2)O + phosphoenolpyruvate + HCO(3)(-)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoenolpyruvate carboxylase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 970 amino acids
Theoretical weight: 109.48 KDa
Source organism: Zea mays
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04711 (Residues: 1-970; Coverage: 100%)
Gene names: PEP1, PPC
Sequence domains: Phosphoenolpyruvate carboxylase
Structure domains: Phosphoenolpyruvate/pyruvate domain

Ligands and Environments

2 bound ligands:
Ligand GLY 5 x GLY
Ligand ACT 13 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 156.95Å b: 167.24Å c: 242.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

2 citation in other articles

Insights into the proteomic profile of newly harvested corn and metagenomic analysis of the broiler intestinal microbiota.
Yin et al. (2022)
1 more

5 mentions without citation

Overexpression of an Agave Phosphoenolpyruvate Carboxylase Improves Plant Growth and Stress Tolerance.
Liu et al. (2021)
4 more