› 5w0k

X-ray diffraction
3.1Å resolution

Crystal structure of EBV gHgL/CL40/gp42 N-domain

Released:
DOI: 10.2210/pdb5w0k/pdb
PDB entry 5w0k coloured by chain, front view.
PDB entry 5w0k coloured by chain, side view.
PDB entry 5w0k coloured by chain, top view.
Source organisms:
Primary publication:
Inhibition of EBV-mediated membrane fusion by anti-gHgL antibodies.
Sathiyamoorthy K, Jiang J, Möhl BS, Chen J, Zhou ZH, Longnecker R, Jardetzky TS
Proc Natl Acad Sci U S A 114 E8703-E8710 (2017)
PMID: 28939750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-230764 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Envelope glycoprotein H Chains: A, C
Molecule details ›
Chains: A, C
Length: 660 amino acids
Theoretical weight: 73.23 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03231 (Residues: 20-679; Coverage: 96%)
Gene names: BXLF2, gH
Sequence domains:
Structure domains: Herpesvirus glycoprotein H, domain D-II
Envelope glycoprotein L Chains: B, D
Molecule details ›
Chains: B, D
Length: 114 amino acids
Theoretical weight: 12.59 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P03212 (Residues: 24-137; Coverage: 99%)
Gene names: BKRF2, gL
Sequence domains: Viral glycoprotein L
Structure domains: Viral glycoprotein L
CL40 IgG heavy chain Chains: E, H
Molecule details ›
Chains: E, H
Length: 217 amino acids
Theoretical weight: 23.35 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
CL40 IgG light chain Chains: F, L
Molecule details ›
Chains: F, L
Length: 213 amino acids
Theoretical weight: 23.6 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Soluble gp42 Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 35 amino acids
Theoretical weight: 4.09 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03205 (Residues: 47-81; Coverage: 16%)
Gene name: BZLF2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 96.38Å b: 133.13Å c: 254.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.299
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus
  • Not provided

Quick links

Citations

4 review citations

The structural basis of herpesvirus entry.
Connolly et al. (2021)
3 more

5 mentions without citation

How EBV Infects: The Tropism and Underlying Molecular Mechanism for Viral Infection.
Bu et al. (2022)
4 more