5w2m

X-ray diffraction
3.7Å resolution

APOBEC3F Catalytic Domain Complex with a Single-Stranded DNA

Released:

Function and Biology Details

Reaction catalysed:
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-119321 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA dC->dU-editing enzyme APOBEC-3F Chains: A, B, C, D, J, K, L, M
Molecule details ›
Chains: A, B, C, D, J, K, L, M
Length: 184 amino acids
Theoretical weight: 22.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IUX4 (Residues: 190-373; Coverage: 49%)
Gene name: APOBEC3F
Sequence domains: APOBEC-like N-terminal domain
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chains: E, N
Molecule details ›
Chains: E, N
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P1
Unit cell:
a: 68.194Å b: 108.489Å c: 108.549Å
α: 79.6° β: 71.8° γ: 71.75°
R-values:
R R work R free
0.25 0.25 0.267
Expression systems:
  • Escherichia coli
  • Not provided