› 5w46

X-ray diffraction
1.18Å resolution

Structure of S65D Phosphomimetic Ubiquitin Refined at 1.2 Angstroms Resolution

Released:
DOI: 10.2210/pdb5w46/pdb
PDB entry 5w46 coloured by chain, front view.
PDB entry 5w46 coloured by chain, side view.
PDB entry 5w46 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Impact of different ionization states of phosphorylated Serine-65 on ubiquitin structure and interactions.
Kazansky Y, Lai MY, Singh RK, Fushman D
Sci Rep 8 2651 (2018)
PMID: 29422536

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143231 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG47 (Residues: 153-228; Coverage: 33%)
Gene name: UBB
Sequence domains: Ubiquitin family

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 33.456Å b: 48.248Å c: 41.707Å
α: 90° β: 99.03° γ: 90°
R-values:
R R work R free
0.179 0.178 0.19
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Structural and functional consequences of NEDD8 phosphorylation.
Stuber et al. (2021)
3 more

1 mention without citation

Impact of different ionization states of phosphorylated Serine-65 on ubiquitin structure and interactions.
Kazansky et al. (2018)