› 5w4s

Solution NMR

Solution structure of C2 domain from protein kinase C alpha in ternary complex with calcium and V5-pHM peptide

Released:
DOI: 10.2210/pdb5w4s/pdb
PDB entry 5w4s coloured by chain, ensemble of 20 models, front view.
PDB entry 5w4s coloured by chain, ensemble of 20 models, side view.
PDB entry 5w4s coloured by chain, ensemble of 20 models, top view.
Source organism: Rattus norvegicus
Primary publication:
Structural Basis of Protein Kinase Cα Regulation by the C-Terminal Tail.
Yang Y, Shu C, Li P, Igumenova TI
Biophys J 114 1590-1603 (2018)
PMID: 29642029

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138746 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein kinase C alpha type Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 16.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05696 (Residues: 155-293; Coverage: 21%)
Gene names: Pkca, Prkca
Sequence domains: C2 domain
Structure domains: C2 domain
Protein kinase C alpha type Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.3 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P05696 (Residues: 649-658; Coverage: 2%)
Gene names: Pkca, Prkca

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 1%
Refinement method: DGSA-distance geometry simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Uncovering Membrane-Bound Models of Coagulation Factors by Combined Experimental and Computational Approaches.
Ohkubo et al. (2021)
2 other articles

1 mention without citation

Structural Basis of Protein Kinase Cα Regulation by the C-Terminal Tail.
Yang et al. (2018)