› 5w5t

X-ray diffraction
1.76Å resolution

Agrobacterium tumefaciens ADP-Glucose Pyrophosphorylase bound to activator ethyl pyruvate

Released:
DOI: 10.2210/pdb5w5t/pdb
PDB entry 5w5t coloured by chain, front view.
PDB entry 5w5t coloured by chain, side view.
PDB entry 5w5t coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Primary publication:
Structural analysis reveals a pyruvate-binding activator site in the Agrobacterium tumefaciens ADP-glucose pyrophosphorylase.
Hill BL, Mascarenhas R, Patel HP, Asencion Diez MD, Wu R, Iglesias AA, Liu D, Ballicora MA
J Biol Chem 294 1338-1348 (2019)
PMID: 30401744

Function and Biology Details

Reaction catalysed: 
ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153925 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-1-phosphate adenylyltransferase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, T, Z
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, T, Z
Length: 415 amino acids
Theoretical weight: 46.47 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P39669 (Residues: 6-420; Coverage: 99%)
Gene name: glgC
Sequence domains: Nucleotidyl transferase

Ligands and Environments

3 bound ligands:
Ligand SO4 75 x SO4
Ligand GOL 46 x GOL
Ligand 9X7 10 x 9X7
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 93.781Å b: 140.145Å c: 228.855Å
α: 107.81° β: 101.84° γ: 90.06°
R-values:
R R work R free
0.166 0.163 0.207
Expression system: Escherichia coli K-12

Quick links

Citations

1 review citation

Structure, function, and evolution of plant ADP-glucose pyrophosphorylase.
Figueroa et al. (2022)
4 other articles

1 mention without citation

Structural analysis reveals a pyruvate-binding activator site in the Agrobacterium tumefaciens ADP-glucose pyrophosphorylase.
Hill et al. (2019)