› 5wb1

X-ray diffraction
3.51Å resolution

Ligand-free US28 with stabilizing intracellular nanobody

Released:
DOI: 10.2210/pdb5wb1/pdb
PDB entry 5wb1 coloured by chain, front view.
PDB entry 5wb1 coloured by chain, side view.
PDB entry 5wb1 coloured by chain, top view.
Source organism: Lama glama
Primary publication:
Viral GPCR US28 can signal in response to chemokine agonists of nearly unlimited structural degeneracy.
Miles TF, Spiess K, Jude KM, Tsutsumi N, Burg JS, Ingram JR, Waghray D, Hjorto GM, Larsen O, Ploegh HL, Rosenkilde MM, Garcia KC
Elife 7 (2018)
PMID: 29882741

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159572 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G-protein coupled receptor homolog US28 Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 51.83 KDa
Source organism: Lama glama
Expression system: Homo sapiens
UniProt:
  • Canonical: P69333 (Residues: 1-310; Coverage: 88%)
Gene name: US28
Sequence domains: 7 transmembrane receptor (rhodopsin family)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 77.6Å b: 38.3Å c: 99.8Å
α: 90° β: 107.4° γ: 90°
R-values:
R R work R free
0.247 0.242 0.289
Expression system: Homo sapiens

Quick links

Citations

8 review citations

Advances in therapeutic peptides targeting G protein-coupled receptors.
Davenport et al. (2020)
7 more

6 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
5 more