› 5wcb

Electron Microscopy
6Å resolution

Katanin hexamer in the ring conformation

Released:
DOI: 10.2210/pdb5wcb/pdb
PDB entry 5wcb coloured by chain, front view.
PDB entry 5wcb coloured by chain, side view.
PDB entry 5wcb coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Katanin spiral and ring structures shed light on power stroke for microtubule severing.
Zehr E, Szyk A, Piszczek G, Szczesna E, Zuo X, Roll-Mecak A
Nat Struct Mol Biol 24 717-725 (2017)
PMID: 28783150
Related structures: EMD-8796

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152729 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Meiotic spindle formation protein mei-1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 472 amino acids
Theoretical weight: 51.8 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P34808 (Residues: 1-472; Coverage: 100%)
Gene names: T01G9.5, mei-1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 5 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6Å
Relevant EMDB volumes: EMD-8796
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
13 more

6 mentions without citation

Microtubule-severing enzymes: From cellular functions to molecular mechanism.
McNally et al. (2018)
5 more