› 5wcj

X-ray diffraction
1.7Å resolution

Crystal Structure of Human Methyltransferase-like protein 13 in complex with SAH

Released:
DOI: 10.2210/pdb5wcj/pdb
PDB entry 5wcj coloured by chain, front view.
PDB entry 5wcj coloured by chain, side view.
PDB entry 5wcj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates.
Jakobsson ME, Małecki JM, Halabelian L, Nilges BS, Pinto R, Kudithipudi S, Munk S, Davydova E, Zuhairi FR, Arrowsmith CH, Jeltsch A, Leidel SA, Olsen JV, Falnes PØ
Nat Commun 9 3411 (2018)
PMID: 30143613

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
eEF1A lysine and N-terminal methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 27.54 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8N6R0 (Residues: 470-699; Coverage: 33%)
Gene names: CGI-01, EEF1AKNMT, FEAT, KIAA0859, METTL13
Sequence domains: Spermine/spermidine synthase domain

Ligands and Environments

2 bound ligands:
Ligand UNX 4 x UNX
Ligand SAH 1 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 50.131Å b: 67.45Å c: 71.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.212
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

Lysine Methylation Regulators Moonlighting outside the Epigenome.
Cornett et al. (2019)
10 more

5 mentions without citation

Chemical Biology of Protein N-Terminal Methyltransferases.
Huang R. (2019)
4 more