› 5wfn

X-ray diffraction
3Å resolution

Revised model of leiomodin 2-mediated actin regulation (alternate refinement of PDB 4RWT)

Released:
DOI: 10.2210/pdb5wfn/pdb
PDB entry 5wfn coloured by chain, front view.
PDB entry 5wfn coloured by chain, side view.
PDB entry 5wfn coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of leiomodin 2 in complex with actin: a structural and functional reexamination
Boczkowska M, Yurtsever Z, Rebowski G, Eck MJ, Dominguez R
Biophys. J. 113 889-899 (2017)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145609 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin-5C Chains: A, B
Molecule details ›
Chains: A, B
Length: 384 amino acids
Theoretical weight: 42.89 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10987 (Residues: 1-376; Coverage: 100%)
Gene names: Act5C, CG4027
Sequence domains: Actin
Structure domains:
Leiomodin-2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 506 amino acids
Theoretical weight: 56.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P5Q4 (Residues: 1-547; Coverage: 91%)
Gene name: LMOD2
Sequence domains: Tropomodulin
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

2 bound ligands:
Ligand ANP 2 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P1
Unit cell:
a: 65.35Å b: 65.65Å c: 81.92Å
α: 101.29° β: 90.94° γ: 107.97°
R-values:
R R work R free
0.21 0.208 0.246
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 citations in other articles

A Third Look at the Structure of Leiomodin Bound to Actin.
Pollard TD. (2017)