› 5wj2

X-ray diffraction
2.41Å resolution

Crystal structure of the green fluorescent protein Clover

Released:
DOI: 10.2210/pdb5wj2/pdb
PDB entry 5wj2 coloured by chain, front view.
PDB entry 5wj2 coloured by chain, side view.
PDB entry 5wj2 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Crystal Structure of Green Fluorescent Protein Clover and Design of Clover-Based Redox Sensors.
Campbell BC, Petsko GA, Liu CF
Structure 26 225-237.e3 (2018)
PMID: 29307487

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.22 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR2 2 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 53.063Å b: 135.176Å c: 168.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.217
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Protein-like Enwrapped Perylene Bisimide Chromophore as a Bright Microcrystalline Emitter Material.
Schmidt et al. (2019)
5 more

3 mentions without citation

mGreenLantern: a bright monomeric fluorescent protein with rapid expression and cell filling properties for neuronal imaging.
Campbell et al. (2020)
2 more