› 5wk3

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN CCL17 AND M116 FAB

Released:
DOI: 10.2210/pdb5wk3/pdb
PDB entry 5wk3 coloured by chain, front view.
PDB entry 5wk3 coloured by chain, side view.
PDB entry 5wk3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into chemokine CCL17 recognition by antibody M116.
Teplyakov A, Obmolova G, Gilliland GL
Biochem Biophys Rep 13 27-31 (2018)
PMID: 29264403

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231405 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
M116 LIGHT CHAIN Chains: P, R, T, V
Molecule details ›
Chains: P, R, T, V
Length: 220 amino acids
Theoretical weight: 24.07 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
M116 HEAVY CHAIN Chains: Q, S, U, W
Molecule details ›
Chains: Q, S, U, W
Length: 230 amino acids
Theoretical weight: 24.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
C-C motif chemokine 17 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 71 amino acids
Theoretical weight: 8.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92583 (Residues: 24-94; Coverage: 100%)
Gene names: CCL17, SCYA17, TARC
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 51.51Å b: 81.93Å c: 130.5Å
α: 93.96° β: 99.21° γ: 104.19°
R-values:
R R work R free
0.181 0.18 0.231
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

1 review citation

Relationship between chemokines and T lymphocytes in the context of respiratory allergies (Review).
Berghi et al. (2020)
2 other articles

3 mentions without citation

The marriage of chemokines and galectins as functional heterodimers.
von Hundelshausen et al. (2021)
2 more