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› 5wk9

X-ray diffraction
1.98Å resolution

R186AP450cam with CN and camphor

Released:
DOI: 10.2210/pdb5wk9/pdb
Model geometry
Fit model/data
PDB entry 5wk9 coloured by chain, front view.
PDB entry 5wk9 coloured by chain, side view.
PDB entry 5wk9 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Effect of Redox Partner Binding on Cytochrome P450 Conformational Dynamics.
Batabyal D, Richards LS, Poulos TL
J Am Chem Soc 139 13193-13199 (2017)
PMID: 28823160

Function and Biology Details

Reaction catalysed: 
2 reduced [2Fe-2S]-[putidaredoxin] + (1R,4R)-camphor + O2 + 2 H(+) =(1R,4R,5R)-5-hydroxycamphor + 2 oxidized [2Fe-2S]-[putidaredoxin] + H2O.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 46.6 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P00183 (Residues: 1-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
Ligand CYN 1 x CYN
Ligand CAM 1 x CAM
Ligand K 1 x K
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P21
Unit cell:
a: 56.95Å b: 58.86Å c: 57.28Å
α: 90° β: 104.99° γ: 90°
R-values:
R R work R free
0.167 0.163 0.215
Expression system: Escherichia coli

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Citations

4 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
3 more