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› 5wlh

X-ray diffraction
1.8Å resolution

Crystal structure of LbaCas13a H328A (C2c2) bound to pre-crRNA (24-nt spacer)

Released:
DOI: 10.2210/pdb5wlh/pdb
Model geometry
Fit model/data
PDB entry 5wlh coloured by chain, front view.
PDB entry 5wlh coloured by chain, side view.
PDB entry 5wlh coloured by chain, top view.
Source organism: Lachnospiraceae bacterium NK4A179
Primary publication:
Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme.
Knott GJ, East-Seletsky A, Cofsky JC, Holton JM, Charles E, O'Connell MR, Doudna JA
Nat Struct Mol Biol 24 825-833 (2017)
PMID: 28892041

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104179 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endoribonuclease Cas13a Chain: A
Molecule details ›
Chain: A
Length: 1440 amino acids
Theoretical weight: 169.13 KDa
Source organism: Lachnospiraceae bacterium NK4A179
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2D0TCH1 (Residues: 1-1440; Coverage: 100%)
pre-crRNA Chain: B
Molecule details ›
Chain: B
Length: 54 nucleotides
Theoretical weight: 17.56 KDa
Source organism: Lachnospiraceae bacterium NK4A179
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand IOD 20 x IOD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 150.842Å b: 92.213Å c: 115.858Å
α: 90° β: 92.425° γ: 90°
R-values:
R R work R free
0.186 0.184 0.217
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

CRISPR-Based Technologies: Impact of RNA-Targeting Systems.
Terns MP. (2018)
24 more

3 mentions without citation

HEPN RNases - an emerging class of functionally distinct RNA processing and degradation enzymes.
Pillon et al. (2021)
2 more