› 5wni

X-ray diffraction
2.65Å resolution

Crystal structure of murine receptor-interacting protein kinase 4 (Ripk4) D143N in complex with ATP

Released:
DOI: 10.2210/pdb5wni/pdb
PDB entry 5wni coloured by chain, front view.
PDB entry 5wni coloured by chain, side view.
PDB entry 5wni coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal Structure of Ripk4 Reveals Dimerization-Dependent Kinase Activity.
Huang CS, Oberbeck N, Hsiao YC, Liu P, Johnson AR, Dixit VM, Hymowitz SG
Structure 26 767-777.e5 (2018)
PMID: 29706531

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-190178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Receptor-interacting serine/threonine-protein kinase 4 Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 38.99 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9ERK0 (Residues: 1-342; Coverage: 44%)
Gene names: Ankrd3, Pkk, Ripk4
Sequence domains: Protein kinase domain
Structure domains: Transferase(Phosphotransferase) domain 1

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: I212121
Unit cell:
a: 71.569Å b: 110.199Å c: 145.324Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.248
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

RIPK protein kinase family: Atypical lives of typical kinases.
Cuny et al. (2021)
1 more

1 mention without citation

Vemurafenib and Dabrafenib Downregulates RIPK4 Level.
Madej et al. (2023)