› 5wpq

Electron Microscopy
3.64Å resolution

Cryo-EM structure of mammalian endolysosomal TRPML1 channel in nanodiscs in closed I conformation at 3.64 Angstrom resolution

Released:
DOI: 10.2210/pdb5wpq/pdb
PDB entry 5wpq coloured by chain, front view.
PDB entry 5wpq coloured by chain, side view.
PDB entry 5wpq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of mammalian endolysosomal TRPML1 channel in nanodiscs.
Chen Q, She J, Zeng W, Guo J, Xu H, Bai XC, Jiang Y
Nature 550 415-418 (2017)
PMID: 29019981
Related structures: EMD-8881

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189325 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Mucolipin-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 592 amino acids
Theoretical weight: 66.66 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q99J21 (Residues: 1-580; Coverage: 100%)
Gene names: Mcoln1, Trpml1
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.64Å
Relevant EMDB volumes: EMD-8881
Expression system: Homo sapiens

Quick links

Citations

36 review citations

Single-particle cryo-EM-How did it get here and where will it go.
Cheng Y. (2018)
35 more

4 mentions without citation

Structure of mammalian endolysosomal TRPML1 channel in nanodiscs.
Chen et al. (2017)
3 more