› 5wu2

X-ray diffraction
2.95Å resolution

Crystal structure of human Tut1 bound with BaUTP, form I

Released:
DOI: 10.2210/pdb5wu2/pdb
PDB entry 5wu2 coloured by chain, front view.
PDB entry 5wu2 coloured by chain, side view.
PDB entry 5wu2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of U6 snRNA-specific terminal uridylyltransferase.
Yamashita S, Takagi Y, Nagaike T, Tomita K
Nat Commun 8 15788 (2017)
PMID: 28589955

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190634 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Speckle targeted PIP5K1A-regulated poly(A) polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 573 amino acids
Theoretical weight: 62.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H6E5 (Residues: 141-874; Coverage: 65%)
Gene names: RBM21, TUT1
Sequence domains: Cid1 family poly A polymerase

Ligands and Environments

3 bound ligands:
Ligand UTP 2 x UTP
Ligand BA 2 x BA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 77.33Å b: 88.4Å c: 184.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.246 0.294
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The life of U6 small nuclear RNA, from cradle to grave.
Didychuk et al. (2018)
9 more

1 mention without citation

Crystal structures of U6 snRNA-specific terminal uridylyltransferase.
Yamashita et al. (2017)