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5wux

X-ray diffraction
2.9Å resolution

TNFalpha-certolizumab Fab

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-208420 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
heavy Chains: A, C, H
Molecule details ›
Chains: A, C, H
Length: 224 amino acids
Theoretical weight: 23.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpB1GAP_S
Structure domains: Immunoglobulins
light Chains: B, D, L
Molecule details ›
Chains: B, D, L
Length: 214 amino acids
Theoretical weight: 23.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
Structure domains: Immunoglobulins
Tumor necrosis factor, soluble form Chains: E, F, G

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: C2
Unit cell:
a: 148.592Å b: 207.219Å c: 112.631Å
α: 90° β: 118.81° γ: 90°
R-values:
R R work R free
0.228 0.226 0.266
Expression systems:
  • Escherichia coli-Pichia pastoris shuttle vector pPpB1GAP_S
  • Escherichia coli-Pichia pastoris shuttle vector pPpARG4