› 5wvg

X-ray diffraction
2.69Å resolution

Crystal structure of a mutant insect group III chitinase complex with (GlcNAc)5 (CAD1-E647L-(GlcNAc)5 ) from Ostrinia furnacalis

Released:
DOI: 10.2210/pdb5wvg/pdb
PDB entry 5wvg coloured by chain, front view.
PDB entry 5wvg coloured by chain, side view.
PDB entry 5wvg coloured by chain, top view.
Source organism: Ostrinia furnacalis
Primary publication:
The deduced role of a chitinase containing two nonsynergistic catalytic domains.
Liu T, Zhu W, Wang J, Zhou Y, Duan Y, Qu M, Yang Q
Acta Crystallogr D Struct Biol 74 30-40 (2018)
PMID: 29372897

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197558 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 52.41 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella phaffii CBS 7435
UniProt:
  • Canonical: U5LUV7 (Residues: 528-987; Coverage: 48%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 71.362Å b: 71.362Å c: 175.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.237
Expression system: Komagataella phaffii CBS 7435

Quick links

Citations

2 citation in other articles

A chitinase with two catalytic domains is required for organization of the cuticular extracellular matrix of a beetle.
Noh et al. (2018)
1 more

1 mention without citation

The deduced role of a chitinase containing two nonsynergistic catalytic domains.
Liu et al. (2018)